Roti®ZOL RNA for molecular biology
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Opis
ROTI®ZOL RNA, 100 ml
Storage temp. +4 °C
ADR 8 III
WGK 2
UN-Nr. 1760
1 ml ROTI®ZOL RNA is used for RNA isolation from 100 mg tissue or 107 cells, respectively.
ROTI®ZOL RNA ready-to-use, for molecular biology
ROTI®ZOL
RNA is a ready-to-use, green-coloured solution for isolating total RNA from
biological samples such as cells or tissue.
ROTI®ZOL
RNA is based on the phenolic isolation procedure known from Chomczynski and
Sacchi (Anal.
Biochem. (1987) 162:156-9). Due to the advanced and
optimized formulation, ROTI®ZOL
RNA results in very high recovery rates of highly pure total RNA. Application
is very convenient and preparation of several samples in parallel is finished
in less than one hour. Additionally, the green colour significantly simplifies
the separation of the RNA containing aqueous phase, the DNA containig
interphase, and the protein containing phenolic phase during the
process.
- Easy and very rapid two-step RNA isolation
- High recovery rates of intact, very pure total RNA
- Applicable on a broad range of tissues and on cell types
- The purified RNA is ready for use in standard downstream applications such as RT-PCR
Typical RNA yield from
5x106 cells:
30-35 μg
Typical purity of RNA prepared from
5x106 cells:
2,08 (A260/A280)