ROTI®Prep Plant RNA

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Opis
ROTI®Prep Plant RNA , 10 preparations
Storage temp. +15 to +25 °C
ADR 9 II
WGK 2
UN-Nr. 3316
Preparation of up to 70 μg RNA from 100 mg plant material.
ROTI®Prep Plant RNA for molecular biology
ROTI®Prep
Plant RNA is designed to isolate total RNA from various plant starting
materials.
The isolation is done from
homogenised plant material, which is then treated with one of the lysis buffers
included in the kit to break down even complex plant components. The lysed
sample is then filtered through a centrifugation column, which binds the DNA.
The filtrate is then transferred to a second centrifugation column that binds
the RNA. After several washing and centrifugation steps, the RNA is eluted from
the membrane by the elution buffer and is now ready for use in all subsequent
applications.
- Specially developed for extraction from plant materials
- Preparation by well-known mini spin-column system
- Fast, easy and efficient
- Preparation in approx. 30 min
- Yield up to 70 µg RNA, depending on source material
- Use of DNase not necessary
- Blossoms
- Leaves
- Roots
- Stems
- Wooden parts
- Seeds
- Grains
Lysis Buffer LSR, Lysis
Buffer LSK, Washing Buffer WSA (conc.), Washing Solution WSL (conc.) RNase-free
Water, centrifugation columns DNA (blue), centrifugation columns RNA (violet),
collection tubes, elution tubes.
Ethanol
99.8 % (p.a., Art. no. 9065) is not included in the kit and should be ordered
separately if required.
Wskazówka dotycząca stosowania | Before extraction, the plant material should first be homogenised in a ROTI®SampleLyse tube (Art. No. 1YKA.1), or by means of a mortar and pestle. |