ROTI®Phenol, for the extraction of nucleic acids

Dostępność:

Na zamówienie, realizacja około 2 tyg.

Cena brutto: 172,42 zł

Cena regularna:

172.42

Najniższa cena z 30 dni przed obniżką:

Cena netto: 140,18 zł

Cena regularna:

Najniższa cena z 30 dni przed obniżką:

ilość 100 ml

towar niedostępny

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nr katalogowy: ROTH-0038.1

Opis

ROTI^®Phenol, 100 ml

ready-to-use, for the extraction of nucleic acids

Phenol solutions, ROTI^®Aqua-Phenol, rotifenol

Density (D) 1,05 g/cm³
Flash point (flp) 79 °C
Storage temp. +4 °C
ADR 6.1 III
WGK 2
UN-Nr. 2821

Redistilled, in TE buffer equilibrated phenol, pH 7,5-8,0.
Draw solution from lower phase. Do not shake before use!
Covered with buffer. Store in the dark!

Phenol solutions for nucleic acid isolation must be buffered and adjusted to the correct pH value prior to use. ROTI^®Phenol is a redistilled, in TE buffer equilibrated readytouse phenol.

ROTI^®Phenol ready-to-use, for the extraction of nucleic acids

Phenol solutions for nucleic acid isolation must be buffered and adjusted to the correct pH value prior to use. ROTI^®Phenol is a redistilled, in TE buffer equilibrated readytouse phenol.

ROTI^®Phenol has been successfully tried and tested for isolating nucleic acids in many research laboratories. This quality product for molecular biology replaces the time consuming preparation of phenol and subsequently any exposure to toxic vapours.

Draw solution from lower phase. Do not shake before use!

Addition of 1 volume ROTI^®Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.

Phenolic DNA Isolation
Background and protocol

Mechanism

Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses.
For distinct phase separation, ROTI^®Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl.

Reduce exposure to toxic chemicals
Prepared from phenol of highest purity
Packed under argon for maximum stability
Successfully tried and tested in many research laboratories

ROTI^®Phenol ready-to-use, for the extraction of nucleic acids

Mechanizm

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