ROTI®Garose-His/Ni NTA-Beads, 100 ml,dla biochemii

ROTI®Garose-His/Ni NTA-Beads

Nickel agarose beads, Agarose beads for affinity chromatography, IMAC matrix/nickel charged, Column matrix for protein isolation, IMAC beads

• For isolation of His-tagged proteins by affinity chromatography under reducing conditions

• For batch mode and gravity flow
  

• For the isolation of His-tagged proteins under reducing conditions

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.

• Superior recovery rate of pure His-tagged proteins

The matrix of ROTI®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two exposed histidine tags with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. The tetradentate NTA cross-linker binds his-tagged proteins very efficiently, leading to high recovery rates with minimized nickel bleeding into the eluate.