ROTI®Cell Trypsin/EDTA solution

Dostępność:

Na zamówienie, realizacja około 2 tyg.

Cena brutto: 90,84 zł

Cena regularna:

90.84

Najniższa cena z 30 dni przed obniżką:

Cena netto: 73,85 zł

Cena regularna:

Najniższa cena z 30 dni przed obniżką:

ilość 100 ml

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nr katalogowy: ROTH-1Y1A.1

Opis

ROTI^®Cell Trypsin/EDTA solution (1x)

CELLPURE^® 0.05 % in DPBS, ready-to-use, sterile

Cell dissociation solution

Density (D) 1 g/cm³
Storage temp. -20 °C
Transport temp. cooled

For detachment of adherent cells from tissue and culture surfaces.
Thawing should be done in a +37 °C water bath or overnight at +2 °C to +8 °C. Swirl gently to homogenize.

Sterylne

Trypsin is a protein-cleaving digestive enzyme of the pancreas belonging to the serine proteases, which cleaves lysine and arginine at the C-terminus. Trypsin solutions are used for the separation of cells from tissue or cell monolayers. Thus, they are essential for routine applications in cell culture passaging, for cell culture experiments to obtain an adequate number of cells or for dissociation of primary tissue.
As an alternative to Trypsin, the morecell-protective solution ROTI^®Cell Accutase solution can be used, which is free of mammalian components.

ROTI^®Cell Trypsin/EDTA solution (1x) CELLPURE^® 0.05 % in DPBS, ready-to-use, sterile

This trypsin solution contains EDTA. EDTA is a chelating agent and enhances the ability of trypsin to detach adherent cells. EDTA binds calcium and magnesium, which additionally weakens cell contacts. This favors the hydrolysis of specific peptide bonds by trypsin.

The required trypsin concentration depends primarily on the cell type and the age of the cell culture. For orientation, you will find a complete protocol in the technical info brochure.

After cell dissociation, trypsin should be inhibited. This can be done either by using FCS or trypsin inhibitors.

Informacje techniczne

Enzym	Trypsin from porcine pancreas
Synonim	Cell dissociation reagents
Zastosowanie	Cell dissociation
Wskazówka dotycząca stosowania	Preparation of aliquots recommended to avoid repeated freeze-thaw cycles.

pH value	7.0-7.6
Osmolality	260-340 mOsm/kg
Endotoxins	≤10.0 EU/ml
Sterility	complies