Pepsin do biochemii

Dostępność: Na zamówienie, realizacja około 2 tyg.
Cena brutto: 429,47 zł

Cena regularna:

429.47
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Cena netto: 349,16 zł

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ilość 50 g

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nr katalogowy: ROTH-KK38.1

Opis

Pepsin, 50 g

≥2 000 FIP-U/g, for biochemistry

Molar mass (M) 35000 g/mol
Storage temp. +4 °C
WGK 1
CAS No. 9001-75-6
EG-Nr. 232-629-3

From porcine gastric mucosa. For detection of trichina in meat.
Well soluble in water, stable in aqueous solutions of pH up to 5.5, unstable above pH 6.0

Pepsin ≥2 000 FIP-U/g, for biochemistry

Acidic endopeptidase, the inactive precursor of which, pepsinogen, is produced in the stomach mucosa. Pepsin cleaves proteins preferentially at carboxylic groups of aromatic amino acids such as phenylalanine and tyrosine. Highly active pepsine is preferably used for detection of trichina in pre-digested muscle meat of animals.


Definicja jednostki

1 FIP unit (≈ Ph. Eur. unit) of Pepsin activity will, during incubation at 25 ±0.1 °C for one minute, cause decomposition of haemoglobin to an extent comparable with the colour caused by reaction of 1 micromole of tyrosine with the same dye. 2 000 FIP-Units correspond to 1: 10 000 NF-U and 1: 12 500 BP-U.


Wskazówki dotyczące zastosowania

Irreversible inactivated in alkaline medium.
Solution is stable in temperatures of up to 60 °C, and also in higher concentrations of urea and guanidine.
pH optimum: 1-2.
Isoelectric point: 1.0


Appearance white to clear beige, crystalline or amorphous powder
Specific activity ≥2000 FIP-U/g
pH value (1 % in H2O) 3.0-5.0
Loss on drying ≤5.0 %



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